Title:
OPTIMIZATION PARAMETERS FOR SEED GERMINATION AND MICROPROPAGATION OF TIGER ORCHID, GRAMMATOPHYLLUM SPECIOSUM

Authors:
Zuraida Ab Rahman, Ayu Nazreena Othman, Amirah Balqis Amir Amran and Nur Najwa Arifah Basiron

Abstract:
Micropropagation is one of the ways to conserve a species and the aim of this study is to optimize the seed germination and also micropropagation of tiger orchid (Grammatophyllum speciosum).The first part is to optimize the seed germination of tiger orchid where full-strength MS media supplemented with 30 % sucrose with the addition of 5 mg/L of BAP is the most preferable condition to germinate the seeds of tiger orchids where the germination recorded at 95 % followed by full strength B5 which the germination recorded at 80 %. The optimization for micropropagation for Grammatophyllum speciosum is identified in this study where the medium supplemented with 0.2 % charcoal and 1 mg/L of BAP produce the heaviest PLB (13.5 g) while the medium supplemented with 1mg/L of BAP with no presence of charcoal contributed to the highest number of shoots (95).