Authors:
Zuraida Ab Rahman, Mohd Shukri Mat Ali, Mohd Norfaizal Ghazalli, Khadijah Awang,
Ayu Nazreena Othman, Amirah Balqis Amir Amran and Nurul Qistina Mohamed Bakri |
Abstract:
In vitro leaves of Lepisanthes fruticosa cultured for callusing, then proliferated into somatic
embryos and eventually regenerated plantlets. The optimal treatment for callus induction of
Lepisanthes fruticose is 1.0 mg/L NAA + 0.2 mg/L 2,4- D. The callus initiation was observed in
medium containing plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) and ?Naphthalene acetic acid (NAA) either on its own or combined. At 0.2 mg/L and 1.0 mg/L of 2,4-
D and NAA, respectively, callus induction showed the highest rate (100%) in WPM media. The
callus showed no sign of browning after sub-cultured. Subculturing the callus onto a medium
with 0.5 mg/L of Kinetin showed the highest proliferation rate (100%) and improved the
regeneration of somatic embryos, which produced the highest number of plantlets (21 plantlets).
BAP with a concentration of 0.5 and 1.0 mg/L increase the browning incidence on the subcultured callus. |
